Recombinant Rat Cathepsin B in Lysosomal Function Studies: What the Protein Data Doesn’t Tell You
Cathepsin B (CTSB) is a lysosomal cysteine protease central to protein degradation, autophagy regulation, and apoptotic signaling. In rat models, it's studied in the context of neurodegeneration, cancer biology, inflammatory disease, and lysosomal storage disorders. Recombinant versions of the protein are used for enzymatic characterization, substrate specificity profiling, inhibitor screening, and pathway reconstitution in cell-free systems.
Working with recombinant rat Cathepsin B in lysosomal function studies requires understanding both the biochemistry of the enzyme and the handling conditions that determine whether your in vitro results are measuring real enzymatic activity or an artifact of preparation.
The Proenzyme Problem Most Protocols Skip
Cathepsin B is synthesized as a pr...